Dr.Sushmita G Shah (Nag Choudhury)
Aim – To evaluate the antifungal efficacy of a novel conjugate, CPP-Natamycin in an experimental model of Fusarium spp. keratitis.
In vivo experimental study
Material & methods – Experimental Fusarium spp. keratitis was established in the right eye of Balb/c mice. The study animals were divided into three groups, each receiving hourly topical instillation of the CPP-Natamycin conjugate (Group 1), Natamycin (Group 2) and phosphate buffer saline (Group 3) for three days. The animals were sacrificed and the right eye was enucleated and evaluated for colony forming units (CFU) using broth dilution technique.
Outcome measure – CFU/ml of Fusarium spp.
Results – Five animals were included in each group. CFU/ml was comparable between Group 1 and Group 2. Group 3 demonstrated the highest CFU/ml.
Conclusion – Compared to Natamycin alone, which is currently the drug of choice in Fusarium spp. keratitis, the novel conjugate CPP – Natamycin demonstrated comparable antifungal efficacy.
Leave a Comment