Dr.Sanket Bhatnagar
Purpose:To study molecular changes during structural healing in corneal stroma of cadaver eyes post Lasik and SMILE surgeries with quantitative proteomics.Methods:Cadaveric eyes(n=3)were treated for vision correction of -6D with ablation at 74-75µ and 65-66µ stromal depth for LASIK and SMILE respectively.Total protein from dissected stroma of each eye was extracted 14h post surgery and subjected to quantitative proteomic analysis by mass spectrometry.Results:Around 400 proteins were identified in stroma of LASIK and SMILE eyes.Cell cycle proteins (1433 class) and cytokeratins were higher in SMILE.Extracellular matrix (collagen 6 A1),cellular homeostasis proteins (Annexin A1, A5) were lower compared to LASIK.Lysozyme C, an inflammatory response protein was also lower in SMILE.Conclusion:Quantitative proteomic analysis of stroma post LASIK and SMILE hints at less inflammatory response, faster wound healing and stable extracellular matrix (ECM) in SMILE 14h post surgery compared to LASIK
Leave a Comment